SpotMap – Workflow | Totallab | Supporting your research

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  3. SpotMap – Workflow | Totallab | Supporting your research

SpotMap has a simple 4 step workflow that produces objective and reliable results from a 2D gel vs 2D Western blot in less than 1 hour per image pair. SpotMap makes the analysis of all images simple. You can see what we can do to help you analyse your images in our analysis report. When images are uploaded to the software they are automatically quality checked, giving you instant feedback on your images and confidence in your results. Images are checked using the following criteria: Tools are available in the software to edit the images. Editing images in other software before analysis risks losing data, using the editor in the software allows you to make minor edits without changing the original. Alignment is a unique method that addresses the challenge of comparing two very different spot patterns commonly seen between gels and blots. It allows corresponding spots on each image to be located in the same coordinate space, allowing spots common to both 2D gel and Western blot to be easily identified. Alignment is performed at the pixel level to provide direct and accurate comparison of images. Alignment is completed automatically or manually. Manual vectors are added to assist alignment of images where large positional differences exist. Align the blot image (green) to the gel image (magenta). Check your alignment using the different viewing modes. A spot map is created of the fully resolved host cell proteins separated in the 2D gel. The spot map is then overlaid on the Blot where presence or absence of identified spots is recorded to calculate anti-HCP coverage relative to the gel. Automatic spot detection creates an initial spot map of 1000’s of spots quickly and objectively in less than 30 seconds. The automatically detected spot map is manually edited to ensure accuracy using the available tools: The automatically detected spots are edited to create a master spot map which represents the position of all spots on the 2D gel. Because your images are aligned, the master spot map can be applied directly to the blot. Spots common to both Gel and Blot will be present in the same coordinate space. Presence of spots on the blot is recorded as unique to gel, unique to blot or common to calculate coverage. The summary page displays the results of the analysis and allows annotation of the spot map with easy export of data directly into reports and presentations. The summary screen allows you to highlight spots and annotate the spot map. All data can be exported using the data access feature. Source. Looking for vector maps of Germany (Deutschland Vektorkarten) for Adobe Illustrator?.”

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