Gene Construction Kit provides many different ways for you to annotate your construct’s appearance and mark important features. You can quit now, or continue on to Tutorial 6: Cloning a DNA Segment and Silent Mutations. If you plan on continuing, you can leave the file you just created, construct#5 , open. If not, close this file. 1. A frame can be selected by option-clicking (on the Mac) on the frame or by choosing Construct >, Select Frame and then clicking on the frame you want to select. For those of you who are “quick-fingered” you can also select a frame by triple-clicking on the DNA contained within a frame. 2. Note that you could accomplish the same result by clicking on a single site marker and then holding down the shift key and clicking on every other site marker except the BamHI site. You may use these HTML tags and attributes: <,a href=” title=”>, <,abbr title=”>, <,acronym title=”>, <,b>, <,blockquote cite=”>, <,cite>, <,code>, <,del datetime=”>, <,em>, <,i>, <,q cite=”>, <,strike>, <,strong>, Source.
